Such a combined BLI-SERS-MD method provides an in-depth understanding of the theophylline-aptamer binding processes and a comprehensive explanation for
Thus, an understanding of what occurs when aptamers bind to theophylline is critical for identifying a high-affinity and high-specificity aptamer, which improve the
fi uorescence of QDs will be quenched in the presence of GO due to the noncovalent assembly between fl ssRNA aptamer and GO, leading to uorescence resonance energy
Overall, 8‐oxoG can have an impact on the structure of aptamers in a position dependent manner, leading to altered target selectivity
Using the structure-switching aptamer sensor design method, a detection limit of 17 nM
In the aptamer field,[9,11,16–17] the theophylline RNA aptamer reported by Jenison et al in 1994 is probably the most mentioned example to articulate the high selectivity of
Modifying the aptamer at position‐G11 led to binding inhibition, however modification at G25 changed the selectivity towards theobromine (Kd ~ 160 μM), while displaying a poor affinity for Since the aptamers differ in only one position, the replacement of the theophylline aptamer with the 3-methylxanthine aptamer was straightforward and led to a 3-methylxanthine-sensitive ligRNA − Thus, an understanding of what occurs when aptamers bind to theophylline is critical for identifying a high-affinity and high-specificity aptamer, which improve the sensitivity and selectivity of theophylline detection
Aptamers are oligonucleotide or peptide molecules that can bind to a specific target molecule (Min et al
A new split aptamer sensing platform is developed for highly sensitive and selective detection of theophylline based on single molecule photobleaching (SMPB) technique
Here 7,8- dihydro-8-oxoguanosine (8-oxoG) has been used, due in part to its unique H-bonding capabilities Etymology
Experimental methods have not revealed at atomic resolution the conformations that the theophylline aptamer explores in its unbound state
Aptamers are attractive constructs due to their high affinity/selectivity towards a target
For other tested analogs, the DNA aptamer also showed better selectivity
A nanoparticle-based detection system for theophylline was designed for the first time using an aptamer as a recognition motif and gold nanoparticles as colorimetric reporters
By truncating the bases flanking the 59 nt dexamethasones (DEX) original aptamer
In 2022, this motif appeared four more times from selections using adenosine, ATP, theophylline, and caffeine as targets, suggesting that this aptamer can also bind methylxanthines
The pattern was the same as in buffer, indicating that the aptamer was still functional in the serum sample
We herein isolated four high-quality aptamers for caffeine with dissociation constants ranging from 2